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The Better Solution

"Analysis of urine specimens is the most commonly performed laboratory test in medicine today. Between 32% and 75% of all testing errors in urinalysis occur in the pre-analytical phase." *

Proper collection, transportation, and handling of urine specimens are important to avoid contamination, or deterioration of constituents.  This is where the complete KOVA® Urinalysis System differentiates itself from conventional urinalysis products. It is designed to eliminate significant sources of variation, assuring the right precision and accuracy, test after test. It includes everything the lab needs to standardize critical steps from sample collection to centrifugation, decanting and resuspension of sediment, slide preparation and reporting results.

(*) Bonini P, Plebani M, Ceriotti F, et al. Errors in laboratory medicine. Clin Chem. 2002;48(5):691-698.

What makes the KOVA System components unique?

  • The secure fit of the KOVA Tubes and Caps prevent spillage during specimen collection, transportation, and handling, minimizing biohazardous exposure as well as reducing the risk of aerosol contamination during centrifugation.
  • The unique KOVA Petters assure volume consistent sediment collection and suspension with one-step, contamination-free decanting.
  • The precision manufactured KOVA Glasstic 10 Slides feature specially designed wells for precise volume control, as well counting grids, eliminating variables that commonly interfere with microscopic analysis. Other slide products can only hold one or two samples, have seams between wells that increase the risk of sample cross-contamination, tend to break more easily, or disperse smaller particles to the edges due to adhesion forces, resulting in miscounts.
  • The KOVA system conforms to CLIA (Clinical Laboratory Improvement Amendments) and (CLSI) Clinical and Laboratory Standards Institute Guidelines.

"The technicians favored the use of the KOVA system for several reasons. The KOVA system addresses several factors that contribute to imprecision in manual microscopic examinations of body fluids.”

 Emerson JF, Emerson SS. Evaluation of a standardized procedure for counting microscopic cells in body fluids. J Clin Lab Anal 2005;19:267-275.

How to use the KOVA System

The KOVA System is so easy that an untrained laboratory technician with less than 30 minutes of instruction can obtain the same results as an experienced technologist. It includes specially engineered microscopic slides, sample tubes & caps, custom pipettes or “petters”, collection cups and multi-level urinalysis controls for reliable, simple and efficient urinalysis testing and standardization.

  • Fill the KOVA Tube to 12mL and firmly attach the KOVA Cap
  • Centrifuge at 400 rcf (1500 rpm) for 5 minutes
  • Insert the KOVA Petter firmly and decant
  • 1.0mL of sediment will be trapped by the KOVA Petter
  • Gently resuspend using the KOVA Petter
  • If desired, add 1 drop of KOVA Stain prior to resuspending
Slide Preparation
  • Using the KOVA Petter, transfer the sample to the cut-out notch on the slide chamber
  • Careful addition of samples insures the hygienic handling properties of the KOVA System
Sediment Suspension
  • 6.6 µL of the sample will be drawn into the KOVA Slide 10 chamber via capillary action resulting in a homogenous suspension of the sediment
  • Quantitate the casts at low power (100x)
  • Quantitate all cells at high power (400x)
  • Count the cells within the lines of the small 0.33 mm square grid (as shown)
  • Refer to the value table for the cell count per µL of patient sample

Counting Cells with the KOVA Glasstic 10 Slides

To download the cell count value tables click here:


Urinalysis Quality Control

Instituting a Quality Assurance program in urinalysis is important to assure accurate and reproducible results.

An internal quality control program can be established using KOVA-Trol to check the performance and reactivity of the lab’s urine chemistry test strips, as well as to monitor any changes occurring in the microscopic sediment analysis. This is accomplished by plotting the reported numbers of KOVA-Trol simulated leukocytes on a day-to-day basis.

If after establishing the mean value and standard deviation limits, a shift is observed; the sources of error can be identified and corrected. Technician variation is eliminated due to the standardization of each step of the KOVA procedure.