Biochemical Diagnostics Material Safety Data Sheets
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Prepare a 0.2M Sodium Acetate Solution. Weigh 27.2 gm of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of Deionized Water. Mix and dissolve. Bring the pH down to 4.0 with Glacial Acetic Acid. Finally adjust the volume to one liter with Deionized Water.
Prepare a 0.2M Sodium Acetate Solution. Weigh 27.2 gm of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of Deionized Water. Mix and dissolve. Bring the pH down to 4.0 with Glacial Acetic Acid. Add 70 mL of n-Propanol. Finally adjust the volume to one liter with Deionized Water and mix.
Prepare a 0.2M Sodium Acetate Solution. Weigh 27.2 gm of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of Deionized Water. Mix and dissolve. Bring the pH down to 5.0 with Glacial Acetic Acid. Finally adjust the volume to one liter with 0.1. water.
Weigh 6.0 gm of Hepes (N-Hydroxyethyl Plperazine N2 Ethanesulfonlc Acid). Dissolve in Deionized Water and bring it up to one liter. Adjust the pH to 8.2 with 2N Sodium Hydroxide.
Prepare 0.25M Potassium Phosphate, Monobasic, (34 gm KH2PO4/liter water). Initial pH is approximately 4.8. Adjust the pH upward to 6.0 with 0.25M Sodium Phosphate, Dibasic , (35.5 gm Na2HPO4/liter of water).
Prepare 0.25M solution of Sodium Phosphate, Dibasic, in Deionized Water. (Initial pH approx. 9.25) Bring the pH down to 8.4 with with the slow addition of a 0.25M solution of Potassium Phosphate, Monobasic, in water.
Prepare a 0.25M solution of Sodium Phosphate Dibasic (Na2HPO4) in Deionized Water. Adjust the pH with Potassium Phosphate Monobasic (KH2PO4)) (if the pH is low use 2N Sodium Hydroxide to bring it up to 9.1).
Prepare as phosphate buffer, pH 9.1 and increase the pH with 2N Sodium Hydroxide to pH 10.0.
Measure 70 mL of n-Propanol and bring it up to one liter with pH 6.0 Phosphate Buffer
Use only Potassium Phosphate Dibasic, ( K2HPO4) because the Sodium Salt will precipitate in n-Propanol. Weigh 43.5 gm of K2HPO4. Mix and dissolve in approx. 900 mL of Deionized Water. Add 70 mL of n-Propanol and adjust the pH to 9.1 with KH2PO4. Bring the volume to one liter with water.
Slowly add 450 gm of Potassium Hydroxide pellets to a volumetric flask containing 500 mL of Deionized Water. This is done in a cool water bath. When all the pellets are dissolved and the temperature has come down to room temperature bring the volume to one liter with Deionized Water. Mix well.
670 gm of Potassium Hydroxide (Assay 88%) is added slowly with cooling and mixing into 400 mL of Deionized Water. When all the solid is dissolved and the solution is at room temperature bring the volume to one liter with water.
Prepare a saturated solution of Sodium Bicarbonate (60 gm/liter Deionized Water). pH should be 8.4. Raise the pH to 9.2 with Anhydrous Sodium Carbonate. Decant the clear supernatant solution and discard the solid at the bottom.
Add 10 grams of Potassium Phosphate Monobasic to 100 mL Deionized Water. Mix will.
Dissolve 5 grams of Sodium Bisulfite (or Sodium Metabisulfite which converts to Sodium Bisulfite in solution) in 50 mL of Deionized water. Shake well. Dilute 1:1 with Phosphate Buffer, pH 6.0 for a total volume of 100mL. Shake well. Prepare weekly and store this reagent in a glass bottle with a Teflon or Polypropylene lined screw capped top.
Dissolve 12.1 grams of TRIS (THAM) base in 900 mL of Deionized Water. Adjust the pH to 9.0 with the slow addition of concentrated HCl.
All solvents should be HPLC grade.
Some drugs are changed when exposed to an oxidizing agent.It is therefore important that Ethyl Acetate (which easily forms peroxides) be peroxide free. Check for peroxides by mixing 1 ml of a 50% (w/v) of Potassium Iodide in water with 9 mL of solvent. A brown color indicates release of Iodine by the oxidative effect of organic peroxides. A drop or two of 0.1 N HCl added to the tube increases the sensitivity of the test.
It is a good idea to screen each new lot # of solvents for contaminants by drying down a few mL of the solvent and following derivatization, if required, injecting it for GC-MS analysis. As an example, we have encountered a contaminant in Acetone which, when used to prepare the 40% wash for THC sample preparation, resulted in a 488 ion contamination at the retention time of THC-COOH. A different lot # of Acetone did not exhibit this contaminant.
Ethyl Acetate: Hexane (1:1, v/v), Acidified with 2 % Glacial Acetic Acid (2 mL Glacial Acetic Acid in 98 mL mixed solvent). Shake well. Prepare monthly and store this reagent in a glass bottle with a Teflon or Polypropylene lined screw capped top.
Solvents or solvent mixes which call for the addition of 2% Triethylamine (TEA) are prepared in a glass bottle with a Teflon or Polypropylene lined screw capped top. 2 ml of TEA is added to 98 mL of the appropriate solvent and mixed well. Prepare fresh reagent bi-monthly.
Preparing 10% HCI (1.2 M)
All solvents should be HPLC grade.
Concentrated HCl is ordinarily supplied as a 12 Molar solution. A 100 mL 1.2 M HCl solution is prepared by carefully adding 10 mL of 12 M acid to 90 mL of water.
PREPARING 1% HCl (0.12 M)
A 100 mL 0.12 M HCl solution is prepared by carefully adding 1.0 mL of 12 M acid to 99 mL of water.
This trouble-shooting guide has been prepared by the technical staff of Biochemical Diagnostics, Inc.
Based on our own experience and the comments of those customers who have responded to our surveys. Our customers have reported very few technical problems when using our complete reagent kits, however, nobody is perfect and occasional problems do occur. In order to minimize startup problems, we ask that you read this guide and the package insert before attempting to set up our tests. If additional information is needed, we encourage you to contact us or leave an e-mail message and we will contact you. Included in this guide are some quality control recommendations for those of you who find it necessary to prepare some of your own reagents. While we discourage this practice, since performance cannot be guaranteed, we have included this information since it is our goal to satisfy every customer. Although our reagents are thoroughly tested for composition purity and performance in our own laboratory, there may be slight variations in the composition of different lot numbers of a particular reagent. Whenever possible, we encourage you to use complete reagent kits in order to obtain optimum performance.
If possible, avoid using different lots of reagents or columns within a single run. When necessary, reagents of different lot numbers should be "pooled" and used as a homogeneous reagent. For best results, this rule should also be applied to reagents of the same lot number.
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BCD Drug Applications
PARTIAL LIST OF DRUGS AND NON-DRUG ANALYTES THAT CAN BE EXTRACTED USING DETECTABUSE® OR MULTI-PREP® COLUMNS DRUGS
The extraction methods listed below are suitable for GC/MS and LC/MS. For LC/MS it is best to substitute ammonia for TEA and either dry down the sample prior to taking it up in mobile phase, or, modify the elution solvent, (e.g. basic methanol or water methanol mixtures), to insure that it is compatible with the mobile phase.Prevention of cross contamination and carryover has always been important but as methods become more sensitive this issue has come to the forefront of laboratory inspections and evaluations. Please visit the "SPE and Sample Preparation" product section see how our hardware design and methods are designed to help you overcome this problem.
The water wash following methanol column activation is optional and applies to columns activated but not used for more than 20 minutes such as might happen when used on a robotics platform Such delays may lead to methanol evaporation which can cause a loss in column efficiency.
Before setting up any of the following extraction procedures we suggest that you become familiar with our troubleshooting guides found in the BCD resources/ BCD support section.
Where to Order (USA)
Biochemical Diagnostics, Inc. 180 Heartland Blvd. Edgewood, NY 11717 New York Phone: 1-800-223-4835 -or- 1-631-595-9200 Fax: 1-631-595-9204 E-mail: firstname.lastname@example.org